Nanopareil produces cutting-edge separation media composed of functionalized nanofibers.
The product is manufactured from a randomly overlaid mat of electrospun nanofibers, which yields a unique separation matrix capable of purifying gene therapy targets with high efficiency.
Market leading binding capacity due to large accessible surface area
- High specific surface area (>1000 m2/g), >80% porous
- No internal diffusion for adsorption = rapid binding kinetics
- < 1s residence time required to obtain saturation capacities
High flow rate with low pressure due to open matrix
- Average ~1.5 µm “effective” pore size with 0.5-2.0 µm range
- Porosity and pore sizes can be varied to fit different applications
Resin-based chromatography drawbacks for large modalities
Purification of AAV, lentivirus, exosomes, plasmid DNA, and mRNA is more difficult with legacy resin technology.
- Difficult to access pores
- Slow diffusion causes slow process time
- Low binding capacities & low resolution
- Oversized columns
AS COMPARED TO RESIN-BASED CHROMATOGRAPHY:
Superior attributes for gene therapy purification
|Nanopareil||Resin Beads||Membrane Absorber||Monolith Tech|
|Large open pore structure||Small pore sizes||Medium-large pore sizes||Large pore sizes|
|Uniform flow distribution||Uniform flow distribution||Uneven flow distribution||Uniform flow distribution|
|High capacity||Medium capacity||Very Low capacity||Low capacity|
(1 sec residence time)
(4-6 min residence time)
|High permeability||High permeability|
Achieve orders of magnitude higher capacity with lightning fast cycle times
>300 g/L binding with a 1 second residence time (60 MV/min)
A Nanopareil Q (quaternary amine) capsule containing 0.1 mL wetted membrane by volume was loaded with 1.0 mg/mL Bovine serum albumin (BSA) to 10% breakthrough. Buffer conditions were 20 mM NaPi, pH 7.0.
The same method was performed using a commercially available Q membrane absorber with similar size.
MV = membrane volumes
Nanopareil technology creates a common purification platform for the next generation of advanced therapies
Nanopareil’s composite nanofibers can be functionalized with a wide variety of chemistries to enable a platform technology capable of accommodating affinity, ion exchange, and hydrophobic interaction chromatography.
We are developing products designed to deliver significant leaps in performance and production efficiency for advanced therapy purification.