Technology

Nanopareil produces cutting-edge separation media composed of functionalized nanofibers.


The product is manufactured from a randomly overlaid mat of electrospun nanofibers, which yields a unique separation matrix capable of purifying gene therapy targets with high efficiency.

Composite nanofibers

Market leading binding capacity due to large accessible surface area


  • High specific surface area (>1000 m2/g), >80% porous
  • No internal diffusion for adsorption = rapid binding kinetics
  • < 1s residence time required to obtain saturation capacities

Fibers woven into a hybrid mat (different fiber types assembled within an overall structure)

High flow rate with low pressure due to open matrix


  • Average ~1.5 µm “effective” pore size with 0.5-2.0 µm range
  • Porosity and pore sizes can be varied to fit different applications
virus

Resin-based chromatography drawbacks for large modalities


Purification of AAV, lentivirus, exosomes, plasmid DNA, and mRNA is more difficult with legacy resin technology.

Resin Challenges

  • Difficult to access pores
  • Slow diffusion causes slow process time
  • Low binding capacities & low resolution
  • Oversized columns

AS COMPARED TO RESIN-BASED CHROMATOGRAPHY:

Superior attributes for gene therapy purification

Nanopareil Nanopareil Resin Beads Resin Beads Membrane Absorber Membrane Absorber Monolith Tech Monolith Tech
Large open pore structure Small pore sizes Medium-large pore sizes Large pore sizes
Uniform flow distribution Uniform flow distribution Uneven flow distribution Uniform flow distribution
High capacity Medium capacity Very Low capacity Low capacity
High permeability
(1 sec residence time)
Low permeability
(4-6 min residence time)
High permeability High permeability

Achieve orders of magnitude higher capacity with lightning fast cycle times


>300 g/L binding with a 1 second residence time (60 MV/min)

A Nanopareil Q (quaternary amine) capsule containing 0.1 mL wetted membrane by volume was loaded with 1.0 mg/mL Bovine serum albumin (BSA) to 10% breakthrough. Buffer conditions were 20 mM NaPi, pH 7.0.

The same method was performed using a commercially available Q membrane absorber with similar size.

MV = membrane volumes

Nanopareil technology creates a common purification platform for the next generation of advanced therapies


Nanopareil’s composite nanofibers can be functionalized with a wide variety of chemistries to enable a platform technology capable of accommodating affinity, ion exchange, and hydrophobic interaction chromatography.

We are developing products designed to deliver significant leaps in performance and production efficiency for advanced therapy purification.

Discover what we are developing